The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性Biological Activity: The ED50 as determined by a cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with VSV virus is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
% SDS-PAGE. Purity by SDS-PAGE and HPLC: 95%
Endotoxin level is <0.1 ng per µg of IFN gamma.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成Reconstitute in ddH2O to a concentration of 1 mg/ml. Further dilutions should be made into RPMI + 10% FCS and stored at 4°C for 1 week or –20°C for future use.
機能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
組織特異性Released primarily from activated T lymphocytes.
関連疾患In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
配列類似性Belongs to the type II (or gamma) interferon family.
翻訳後修飾Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.