Recombinant mouse IL-17AF protein (ab109674)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, Functional Studies
製品の詳細
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製品名
Recombinant mouse IL-17AF protein -
生理活性
The activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts, is typically 75-325 ng/mL.
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精製度
> 98 % SDS-PAGE.
Purity Greater than 98% determined by reducing and non-reducing SDS-PAGE. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
IL-17A subunit: MAAIIPQSSACPNTEAKDFLQNVKVNLKVFNSLGAKVSSRRPSDYLNRST SPWTLHRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILV LKREPESCPFTFRVEKMLVGVGCTCVASIVRQAA
IL-17F subunit: MRKNPKAGVPALQKAGNCPPLEDNTVRVDIRIFNQNQGISVPREFQNRSS SPWDYNITRDPHRFPSEIAEAQCRHSGCINAQGQEDSTMNSVAIQQEILV LRREP -
予測される分子量
31 kDa -
配列の追加情報
ab109674 is a disulfide-linked heterodimer. It contains one IL-17A subunit and one IL-17F subunit. It contains 271 amino acids and has a molecular weight of 30.7 kDa.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab109674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
Functional Studies
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製品の状態
Lyophilized -
備考
Recombinant mouse IL-17AF is a non-glycosylated, disulfide-linked heterodimer. It contains one IL-17A subunit and one IL-17F subunit, with a total of 271 amino acids and an molecular weight of 30.7 kDa.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended to reconstitute ab109674 with sterile water to a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
関連情報
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関連性
Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17R receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells.
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109674 は論文での使用が確認できていません。