The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts, is typically 75-325 ng/mL.
< 0.100 Eu/µg
% SDS-PAGE. Purity Greater than 98% determined by reducing and non-reducing SDS-PAGE.
Recombinant mouse IL-17AF is a non-glycosylated, disulfide-linked heterodimer. It contains one IL-17A subunit and one IL-17F subunit, with a total of 271 amino acids and an molecular weight of 30.7 kDa.
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Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute ab109674 with sterile water to a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17R receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells.
Other - Active Recombinant Mouse IL-17AF (ab109674)
ab109674 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse IL-17AF is a heterodimer with a predicted total MW of 30.7 kDa.
has not yet been referenced specifically in any publications.