製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号Q9QZR5
    • 生物種Mouse
    • 分子量72 kDa including tags
    • 領域153 to 564

関連製品

特性

Our Abpromise guarantee covers the use of ab125553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The specific activity was determined to be 110 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % Densitometry.

  • 製品の状態Liquid
  • 備考

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • hHIPk 2
    • hHIPk2
    • HIPK 2
    • Hipk2
    • HIPK2_HUMAN
    • Homeodomain interacting protein kinase 2
    • Homeodomain-interacting protein kinase 2
    • Nbak1
    • Nuclear body-associated kinase 1
    • PRO0593
    • Sialophorin tail-associated nuclear serine/threonine-protein kinase
    • Stank
    see all
  • 機能Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
  • 組織特異性Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated on tyrosines (By similarity). Autophosphorylated.
    Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
    Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML.
  • 細胞内局在Nucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
  • Information by UniProt

Recombinant mouse HIPK2 protein 画像

  • ab125553 by SDS-PAGE
  • Kinase activity of ab125553.

Recombinant mouse HIPK2 protein (ab125553) 使用論文

ab125553 has not yet been referenced specifically in any publications.

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