Recombinant mouse GM-CSF protein (ab9742)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Mouse
    • 配列MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
    • 分子量14 kDa

関連製品

特性

Our Abpromise guarantee covers the use of ab9742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態Lyophilised
  • 備考The ED50, determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells, is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • 機能Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 配列類似性Belongs to the GM-CSF family.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant mouse GM-CSF protein (ab9742) 使用論文

ab9742 has not yet been referenced specifically in any publications.

Product Wall

Application Functional Studies
this protein worked fine for the differentiation of mouse bone marrow-derived macrophage cells with lentivirus transduction. we checked its purity via SDS-PAGE as well prior to the experiment, QC was good.
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Abcam user community

Verified customer

投稿 Oct 12 2016

Abreviews
Application Other
We used this rGM-CSF in cell culture to differentiate murine bone marrow cells into bone marrow-derived dendritic cells (BMDCs), and we had a much better yield than when using a competitor's product. In addition, after selection by magnetic beads (negative selection for F4/80, then positive selection for CD11c), we had >90% purity of BMDCs using this rGM-CSF for culture. Finally, using the cells generated from this rGM-CSF, we saw significant anti-fungal activity of these BMDCs in vitro against Cryptococcus neoformans.
Username

Ms. Cheryl Sandoval

Verified customer

投稿 Jun 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"