The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml.
% SDS-PAGE. Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and analytical HPLC.
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Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with sterile water at a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
Reconstituted material should be aliquoted and frozen at -20oC. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Epidermal growth factor
Pro epidermal growth factor
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Expressed in kidney, salivary gland, cerebrum and prostate.
Contains 9 EGF-like domains. Contains 9 LDL-receptor class B repeats.
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
ab72994 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse EGF is predicted to be a homodimer with a MW of 12.4 kDa.
has not yet been referenced specifically in any publications.