The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: 17 units/ul.
Definition of specific activity: 1 unit is defined as the amount of the enzyme that transfer 1 pmole of methyl group to poly dI-dC substrate during 30 minutes at 37°C.
The Dnmt1 Reaction Buffer (5x) and S-adenosylmethionine (SAM) are NOT included. Customers will have to obtain these before starting their experiment.
Incubate in 1 x Dnmt1 Reaction Buffer (20mM Tris-HCl, pH7.4, 0.5 mM EDTA, 0.2 mM DTT, 5% glycerol) with 10µM S-adenosylmethionine (SAM) at 37°C.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
CXXC finger protein 9
CXXC-type zinc finger protein 9
DNA (cytosine 5 ) methyltransferase 1
DNA (cytosine-5)-methyltransferase 1
DNA methyltransferase 1
DNA methyltransferase HsaI
DNA methyltransferase M.HsaI.
DNA MTase HsaI
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Belongs to the C5-methyltransferase family. Contains 2 BAH domains. Contains 1 CXXC-type zinc finger.
The N-terminal part is required for homodimerization and acts as a regulatory domain.