Recombinant mouse Dnmt1 protein (ab91367)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant mouse Dnmt1 protein
Dnmt1 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity: 17 units/ul. Definition of specific activity: 1 unit is defined as the amount of the enzyme that transfer 1 pmole of methyl group to poly dI-dC substrate during 30 minutes at 37°C'
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精製度
> 95 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected insect cells -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
領域
290 to 1620
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab91367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
The Dnmt1 Reaction Buffer (5x) and S-adenosylmethionine (SAM) are NOT included. Customers will have to obtain these before starting their experiment. Reaction Conditions: Incubate in 1 x Dnmt1 Reaction Buffer (20mM Tris-HCl, pH7.4, 0.5 mM EDTA, 0.2 mM DTT, 5% glycerol) with 10µM S-adenosylmethionine (SAM) at 37°C.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 6
Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 1.16% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ADCADN
- AIM
- CXXC finger protein 9
see all -
機能
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. -
組織特異性
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. -
配列類似性
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger. -
ドメイン
The N-terminal part is required for homodimerization and acts as a regulatory domain. -
翻訳後修飾
Sumoylated; sumoylation increases activity. -
細胞内局在
Nucleus. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab91367 は 1 報の論文で使用されています。
- Fattal I et al. Epstein-Barr virus antibodies mark systemic lupus erythematosus and scleroderma patients negative for anti-DNA. Immunology 141:276-85 (2014). PubMed: 24164500