製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Mouse
    • タグGST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab51398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The specific activity was 3214nmol/min/mg in a Kinase activity assay.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態Liquid
  • 備考

    ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Calcium / calmodulin dependent protein kinase 1
    • Calcium / calmodulin dependent protein kinase I
    • Calcium / calmodulin dependent protein kinase type 1
    • Calcium/calmodulin dependent protein kinase 1
    • Calcium/calmodulin dependent protein kinase I
    • Calcium/calmodulin dependent protein kinase type 1
    • Calcium/calmodulin-dependent protein kinase type 1
    • CaM K1
    • CaM KI
    • CAM kinase 1
    • CaM kinase 1 alpha
    • CaM kinase I
    • CaM kinase I alpha
    • CaM-KI
    • CaMK 1
    • CAMK I
    • CAMK1
    • CaMK1 alpha
    • CAMK1 PEN
    • CaMKI alpha
    • CaMKI-alpha
    • KCC1A_HUMAN
    • MGC120317
    • MGC120318
    see all
  • 機能Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
  • 組織特異性Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • 翻訳後修飾Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
    Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic.
  • Information by UniProt

Recombinant mouse CaMKI protein 画像

  • Purity determined to be > 90% by densitometry. Molecular weight 70 kDa.

Recombinant mouse CaMKI protein (ab51398) 使用論文

ab51398 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"