Recombinant mouse Aurora A protein (ab60327)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号P97477
    • 生物種Mouse

特性

Our Abpromise guarantee covers the use of ab60327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity: 69 nmol/min/mg.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • 製品の状態Liquid
  • 備考

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AIK
    • ARK-1
    • ARK1
    • AURA
    • Aurka
    • Aurora 2
    • Aurora A
    • Aurora kinase A
    • Aurora-related kinase 1
    • Aurora/IPL1 like kinase
    • Aurora/IPL1-related kinase 1
    • AURORA2
    • Breast tumor-amplified kinase
    • BTAK
    • hARK1
    • IAK
    • IPL1 related kinase
    • MGC34538
    • OTTHUMP00000031340
    • OTTHUMP00000031341
    • OTTHUMP00000031342
    • OTTHUMP00000031343
    • OTTHUMP00000031344
    • OTTHUMP00000031345
    • OTTHUMP00000166071
    • OTTHUMP00000166072
    • PPP1R47
    • Protein phosphatase 1, regulatory subunit 47
    • Serine/threonine kinase 15
    • Serine/threonine kinase 6
    • Serine/threonine-protein kinase 15
    • Serine/threonine-protein kinase 6
    • Serine/threonine-protein kinase aurora-A
    • STK15
    • STK6
    • STK6_HUMAN
    • STK7
    see all
  • 機能Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • 組織特異性Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt

Recombinant mouse Aurora A protein 画像

  • Sample Kinase Activity Plot.
  • ab60327 on SDS-PAGE, MW ~71kDa.

Recombinant mouse Aurora A protein (ab60327) 使用論文

ab60327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"