Recombinant mouse AHA1 protein (ab111143)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, WB
製品の詳細
-
製品名
Recombinant mouse AHA1 protein
AHA1 タンパク質・ペプチド 製品一覧 -
生理活性
2uM ab111143 generated a 9-fold ATPase activation of 2uM Hsp90 (His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees celcius (this is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
-
精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Mouse -
予測される分子量
38 kDa -
領域
1 to 338 -
タグ
His tag N-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab111143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
Western blot
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 0.002% DTT, 0.378% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Activator of 90 kDa heat shock protein ATPase homolog 1
- Activator of heat shock 90kDa protein ATPase homolog 1
- AHA 1
see all -
機能
Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking. -
組織特異性
Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta. -
配列類似性
Belongs to the AHA1 family. -
細胞内局在
Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab111143 は論文での使用が確認できていません。