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Our Abpromise guarantee covers the use of ab52071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SPECIFIC ACTIVITY: 300,000 units/mg. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C.
Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 5% Glycerol, 50mM Sodium chloride, 50mM HEPES, 0.1% Chaps, 10mM DTT, 10mM EDTA, pH 7.2
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab52071 has not yet been referenced specifically in any publications.