製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      57 kDa including tags
    • 領域
      2 to 497
    • タグ
      DDDDK tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab95933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific Activity: 0.0015 pmol/min/µg Assay conditions: 4 mM ATP, 5mM MgCl2 (both required for reaction), 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotinylated Ubiquitin (1 µg) in 50mM Tris pH 7.4.
  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 精製度
    65 % .
    Purity is >65% by SDS-PAGE.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Store at -80°C.

    pH: 8.00
    Constituents: 20% Glycerol, 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Tween, 0.0462% DTT
    Note: 104 µg/ml FLAG peptide

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AP 13
    • API3
    • Apoptosis Inhibitor 3
    • Baculoviral IAP repeat containing 4
    • Baculoviral IAP Repeat Containing Protein 4
    • Baculoviral IAP repeat-containing protein 4
    • BIRC 4
    • BIRC4
    • E3 ubiquitin-protein ligase XIAP
    • hIAP-3
    • hIAP3
    • HILP
    • IAP 3
    • IAP like protein
    • IAP-3
    • IAP-like protein
    • IAP3
    • ILP
    • ILP 1
    • ILP1
    • Inhibitor of apoptosis protein 3
    • Inhibitor of Apoptosis X Linked
    • Mammalian IAP Homologue A
    • MIHA
    • X linked IAP
    • X linked inhibitor of apoptosis
    • X linked inhibitor of apoptosis E3 ubiquitin protein ligase
    • X linked inhibitor of apoptosis protein
    • X-linked IAP
    • X-linked inhibitor of apoptosis protein
    • Xiap
    • XIAP_HUMAN
    • XLP2
    see all
  • 機能
    Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
  • 組織特異性
    Ubiquitous, except peripheral blood leukocytes.
  • 関連疾患
    Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
  • 配列類似性
    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.
  • ドメイン
    The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
  • 翻訳後修飾
    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt

画像

  • Image showing specific activity of ab95933.
  • SDS-PAGE showing ab95933 at approximately 57kDa (5µg).

参考文献

ab95933 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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