Recombinant Human Werner's syndrome helicase WRN protein (ab112372)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号Q14191
    • 生物種Human
    • 配列NPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNK RRCFPGSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKL MDKTKRGGLFS
    • 分子量38 kDa including tags
    • 領域1322 to 1432

特性

Our Abpromise guarantee covers the use of ab112372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性useful for Antibody Production and Protein Array
  • アプリケーション

    Western blot

    ELISA

    SDS-PAGE

  • 製品の状態Liquid
  • 備考ab112372 is best used within three months from the date of receipt.useful for Antibody Production and Protein Array
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione
    Note: Glutathione is reduced

関連情報

  • 別名
    • DKFZp686C2056
    • DNA helicase
    • DNA helicase, RecQ like type 3
    • Exonuclease WRN
    • HGNC 12791
    • OTTHUMP00000225301
    • RecQ protein-like 2
    • RecQ-like type 3
    • RecQ3
    • RECQL2
    • RECQL3
    • Werner syndrome ATP-dependent helicase
    • Werner syndrome helicase
    • Werner syndrome protein
    • Werner syndrome, RecQ helicase like
    • WRN
    • WRN_HUMAN
    see all
  • 機能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • 関連疾患Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 配列類似性Belongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻訳後修飾Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus. Nucleus.
  • Information by UniProt

Recombinant Human Werner's syndrome helicase WRN protein 画像

  • ab112372 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Recombinant Human Werner's syndrome helicase WRN protein (ab112372) 使用論文

ab112372 has not yet been referenced specifically in any publications.

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