製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号Q99986
    • 生物種Human
    • 分子量71 kDa including tags
    • 領域1 to 396

関連製品

特性

Our Abpromise guarantee covers the use of ab125555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The specific activity of ab125555 was determined to be 2 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • 製品の状態Liquid
  • 備考

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • MGC117401
    • MGC138280
    • MGC142070
    • PCH1
    • PCH1A
    • Serine/threonine protein kinase VRK1
    • Serine/threonine-protein kinase VRK1
    • Vaccinia related kinase 1
    • Vaccinia virus B1R related kinase 1
    • Vaccinia-related kinase 1
    • VRK1
    • VRK1_HUMAN
    see all
  • 機能Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
  • 組織特異性Widely expressed. Highly expressed in fetal liver, testis and thymus.
  • 関連疾患Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
  • 配列類似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
  • 細胞内局在Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • Information by UniProt

Recombinant human VRK1 protein 画像

  • SDS-PAGE analysis of ab125555.
  • Sample Kinase Assay showing the Specific Activity of ab125555 to be 2 nmol/min/mg.

Recombinant human VRK1 protein (ab125555) 使用論文

ab125555 has not yet been referenced specifically in any publications.

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