Recombinant human VRK1 protein (ab125555)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human VRK1 protein -
生理活性
The specific activity of ab125555 was determined to be 2 nmol/min/mg.
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精製度
> 95 % Densitometry.
Purity was determined to be >95% by densitometry. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
71 kDa including tags -
領域
1 to 396
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特性
Our Abpromise guarantee covers the use of ab125555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- MGC117401
- MGC138280
- MGC142070
see all -
機能
Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. -
組織特異性
Widely expressed. Highly expressed in fetal liver, testis and thymus. -
関連疾患
Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA). -
配列類似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. -
細胞内局在
Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab125555 は 1 報の論文で使用されています。
- Cartwright TN et al. Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis. Sci Rep 12:11210 (2022). PubMed: 35778595