Recombinant Human Vimentin protein (ab84704)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 分子量60 kDa
    • 領域1 to 466
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab84704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

  • 精製度> 95 % SDS-PAGE.
    Purity >95% as determined by densitometry.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0

関連情報

  • 別名
    • CTRCT30
    • Epididymis luminal protein 113
    • FLJ36605
    • HEL113
    • VIM
    • VIME_HUMAN
    • Vimentin
    see all
  • 機能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 関連疾患Cataract 30
  • 配列類似性Belongs to the intermediate filament family.
  • ドメインThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻訳後修飾Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 細胞内局在Cytoplasm.
  • Information by UniProt

Recombinant Human Vimentin protein 画像

  • SDS-PAGE of ab84704 using 3ug Vimentin protein. Detected molecular weight approximately 60 kDa.

Recombinant Human Vimentin protein (ab84704) 使用論文

ab84704 has not yet been referenced specifically in any publications.

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