製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 領域5 to 169
    • タグHis tag N-Terminus
    • 配列の追加情報This recombinant VEGF protein comprises a 165 amino acid fragment (5-169) corresponding to the VEGF 165 isoform and is expressed in E. coli with an aminoterminal hexahistidine tag.

特性

Our Abpromise guarantee covers the use of ab55566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 20mM Tris, pH 8

関連情報

  • 別名
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • 機能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 組織特異性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 関連疾患Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性Belongs to the PDGF/VEGF growth factor family.
  • 細胞内局在Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

Recombinant Human VEGFA protein 画像

  • ab55566 on SDS-PAGE

Recombinant Human VEGFA protein (ab55566) 使用論文

This product has been referenced in:
  • Hunter RK  et al. Adipose-Derived Stromal Vascular Fraction Cell Effects on a Rodent Model of Thin Endometrium. PLoS One 10:e0144823 (2015). WB . Read more (PubMed: 26657744) »
  • Mannell HK  et al. ARNO regulates VEGF-dependent tissue responses by stabilizing endothelial VEGFR-2 surface expression. Cardiovasc Res 93:111-9 (2012). Read more (PubMed: 22002459) »

See all 2 Publications for this product

Product Wall

I am sorry my previous email was unclear.

The phosphorylation status of ab42614 is no known. This is not something that is determined during the production process.

I hope this information is helpful.

Thank you for your reply.

Actually it is fine to centrifuge agarose beads. Typically for IP I have centrifuged agarose beads at 500xg for 3-5 min at 4 degrees C.

Regarding your questions 1 and 2, those notes on the datasheet for ab4...

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Thank you for your enquiry.

I think I have found a product that will suit your needs:

http://www.abcam.com/GST-antibody-Agarose-ab90667.html

This is anti-GST antibody covalently linked to agarose beads. Using this product ...

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I'm happy to help! Please contact me again if you need anything further.

Thank you for your enquiry.

VEGF Receptor 2 protein (Tagged) (ab42614) contains an N-terminal GST tag.

I hope this is helpful. Please contact me again if you have any further questions.


I am very pleased to hear you would like to accept our offer and test ab55566 inFunctional Studies.This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview for Functional Studiesand include...

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Thank you very much for your interest in ab55566.

To our knowledge, ab55566 has not been tested in functional assays. Therefore, I can offer a discount off a future purchase if you buy ab55566 now, test it in a functional assayand submit fee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"