Recombinant human VEGF 121B protein (ab86935)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
    • 領域207 to 327

特性

Our Abpromise guarantee covers the use of ab86935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • エンドトキシン・レベル< 1.000 Eu/µg
  • 質量分析
    MALDI-TOF
  • 精製度> 95 % SDS-PAGE.
    purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • MVCD1
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • VEGF
    • VEGF A
    • VPF
    see all
  • 関連性VEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF 121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
  • 細胞内局在Secreted

Recombinant human VEGF 121B protein 画像

  • 15% SDS Page analysis of 3ug ab86935
  • NIH/3T3 cell proliferation was determined by BrdU incorporation method at 405 nm. This test was performed duplicate.N.C. : negative control [only 0.1% FBS]
    P.C. : positive control [10% FBS]

Recombinant human VEGF 121B protein (ab86935) 使用論文

ab86935 has not yet been referenced specifically in any publications.

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