Recombinant Human ULK1 protein (ab95322)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号O75385
    • 生物種Human
    • 分子量113 kDa including tags
    • 領域2 to 1050
    • タグDDDDK tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab95322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 7.5

関連情報

  • 別名
    • ATG 1
    • ATG1
    • ATG1 autophagy related 1 homolog
    • ATG1A
    • Autophagy related protein 1 homolog
    • Autophagy-related protein 1 homolog
    • FLJ38455
    • FLJ46475
    • hATG1
    • KIAA0722
    • Serine/threonine protein kinase ULK1
    • Serine/threonine protein kinase Unc51.1
    • Serine/threonine-protein kinase ULK1
    • ULK 1
    • ULK1
    • ULK1_HUMAN
    • UNC 51
    • Unc 51 (C. elegans) like kinase 1
    • Unc 51 like kinase 1
    • Unc-51 like kinase 1 (C. elegans)
    • Unc-51-like kinase 1
    • UNC51
    • UNC51, C. elegans, homolog of
    • Unc51.1
    see all
  • 機能Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • 組織特異性Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • 細胞内局在Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt

Recombinant Human ULK1 protein 画像

  • 10% SDS-PAGE analysis
    Lane 1: 3 µg ab95322
    Lane 2: Molecular Weight Markers

Recombinant Human ULK1 protein (ab95322) 使用論文

ab95322 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

Attached please find a WB image confirming the protein.

I hope this information is helpful.

Please do not hesitate to contact us if you have any additional questions.

Thank you for contacting Abcam regarding ab95322.

I have confirmed that yes, we have the western blot data to confirm this protein band. Iam working on getting an image from the lab for you. We don’t know the identity of the other band...

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