製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号O75385
    • 生物種Human
    • 分子量125 kDa including tags
    • 領域1 to 649

関連製品

特性

Our Abpromise guarantee covers the use of ab125656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The specific activity of ab125656 was determined to be 50 - 56 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 70 % Densitometry.
    Purity was determined to be >70% by densitometry.
  • 製品の状態Liquid
  • 備考

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • ATG 1
    • ATG1
    • ATG1 autophagy related 1 homolog
    • ATG1A
    • Autophagy related protein 1 homolog
    • Autophagy-related protein 1 homolog
    • FLJ38455
    • FLJ46475
    • hATG1
    • KIAA0722
    • Serine/threonine protein kinase ULK1
    • Serine/threonine protein kinase Unc51.1
    • Serine/threonine-protein kinase ULK1
    • ULK 1
    • ULK1
    • ULK1_HUMAN
    • UNC 51
    • Unc 51 (C. elegans) like kinase 1
    • Unc 51 like kinase 1
    • Unc-51 like kinase 1 (C. elegans)
    • Unc-51-like kinase 1
    • UNC51
    • UNC51, C. elegans, homolog of
    • Unc51.1
    see all
  • 機能Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • 組織特異性Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • 細胞内局在Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt

Recombinant human ULK1 protein 画像

  • SDS-PAGE analysis of ab125656.
  • Sample Kinase Assay showing the specific activity of ab125656 as 50 nmol/min/mg.

Recombinant human ULK1 protein (ab125656) 使用論文

ab125656 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125656.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"