Recombinant Human Ubiquitin protein (Chemical Free) (ab80760)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P0CG47
    • 生物種Human
    • 分子量10 kDa
    • 配列の追加情報N-terminal HA-tagged ubiquitin protein

特性

Our Abpromise guarantee covers the use of ab80760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Conjugation

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • 備考

    This fully functional N-terminal HA-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The HA peptide sequence (YPYDVPDYA) is an epitope derived from the influenza hemagglutinin protein. This tag is specifically recognized by anti-HA antibodies and anti-HA-agarose.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    None

  • 再構成Soluble in aqueous solutions up to 10 mg/ml. Store solubilized stock solution at -20oC. Avoid multiple freeze/thaw cycles.

関連情報

  • 別名
    • FLJ25987
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBB_HUMAN
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • 関連性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 細胞内局在Cell Membrane, Cytoplasmic and Nuclear

Recombinant Human Ubiquitin protein (Chemical Free) (ab80760) 使用論文

This product has been referenced in:
  • Hsiao ES & Tzen JT Ubiquitination of oleosin-H and caleosin in sesame oil bodies after seed germination. Plant Physiol Biochem 49:77-81 (2011). Conjugation . Read more (PubMed: 21041098) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab80760.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"