Recombinant Human Ubiquitin protein (ab57798)



  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human


Our Abpromise guarantee covers the use of ab57798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション


  • 精製度> 95 % SDS-PAGE.
    Supplied essentially salt free.
  • 製品の状態Lyophilised
  • 備考

    Ubiquitin, N-terminal FLAG-tagged, can replace the ubiquitin in formation of poly-ubiquitin-protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blots using anti-FLAG antibodies.

  • Concentration information loading...


  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Buffer: None

  • 再構成Solubility: 10 mg/ml in 50 mM Tris, pH 7.4


  • 別名
    • Epididymis secretory protein Li 50
    • FLJ25987
    • HEL S 50
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • 関連性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 細胞内局在Cell Membrane, Cytoplasmic and Nuclear

Recombinant Human Ubiquitin protein 画像

  • All lanes : Anti-FLAG antibody.

    Lane 1 : Recombinant Human Ubiquitin protein (ab57798) at 0.1 µg
    Lane 2 : Recombinant Human Ubiquitin protein (ab57798) at 0.01 µg

    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 12 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa (possible dimer,post-translational modification).

    This data was provided by an anonymous collaborator

Recombinant Human Ubiquitin protein (ab57798) 使用論文

ab57798 has not yet been referenced specifically in any publications.

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