Our Abpromise guarantee covers the use of ab80794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Conjugation
SDS-PAGE
This ubiquitin mutant contains only a single lysine, K33, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K33 lysine.
Typical concentrations for non rate-limiting support of in vitro conjugation reactions range from 200 μM-1 mM depending on experimental conditions.
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Preservative: None
ab80794 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"