Recombinant Human Ubiquitin (mutated K29) protein (Chemical Free) (ab80818)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P62988
    • 生物種Human
    • 分子量9 kDa
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab80818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Conjugation

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • 備考

    Mutation of lysine 29 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 29 linkages with other Ub molecules. ab80818 can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K29 linked.

    Typical concentrations for non rate-limiting support of in vitro conjugation reactions range from 200 μM-1 mM depending on experimental conditions.

     

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    None

  • 再構成Soluble and stable aqueous buffers up to 5 mg/ml. Store at -20°C solubilization in desired buffer. Avoid multiple freeze/thaw cycles.

関連情報

  • 別名
    • FLJ25987
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBB_HUMAN
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • 関連性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 細胞内局在Cell Membrane, Cytoplasmic and Nuclear

Recombinant Human Ubiquitin (mutated K29) protein (Chemical Free) (ab80818) 使用論文

ab80818 has not yet been referenced specifically in any publications.

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