Recombinant Human Ubiquitin (mutated K29 + K48 + K63) protein (Chemical Free) (ab81669)
製品の概要
- 製品名Recombinant Human Ubiquitin (mutated K29 + K48 + K63) protein (Chemical Free)
Ubiquitin タンパク質・ペプチド 製品一覧 - タンパク質長Full length protein
製品の詳細
- 由来Recombinant
- 由来Escherichia coli
- アミノ酸配列
- アクセッション番号P0CG47
- 生物種Human
- 分子量9 kDa
特性
Our Abpromise guarantee covers the use of ab81669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- アプリケーション
Conjugation
SDS-PAGE
- 精製度> 95
% SDS-PAGE.
- 製品の状態Lyophilised
- 備考
Mutation of lysines 29, 48 and 63 to arginine renders ubiquitin unable to form poly-ubiquitin chains via the major known poly-ubiquitination sites. The ubiquitin triple mutant can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for studies of alternate mono-ubiquitination or polyubiquitination sites.
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
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Concentration information loading...
前処理および保存
- 保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
None
- 再構成Soluble and stable in aqueous buffers up to 5 mg/ml.
関連情報
-
別名
- Epididymis secretory protein Li 50
- FLJ25987
- HEL S 50
see all - 関連性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
- 細胞内局在Cell Membrane, Cytoplasmic and Nuclear
Recombinant Human Ubiquitin (mutated K29 + K48 + K63) protein (Chemical Free) (ab81669) 使用論文
ab81669 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"