Our Abpromise guarantee covers the use of ab81669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Conjugation
SDS-PAGE
Mutation of lysines 29, 48 and 63 to arginine renders ubiquitin unable to form poly-ubiquitin chains via the major known poly-ubiquitination sites. The ubiquitin triple mutant can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for studies of alternate mono-ubiquitination or polyubiquitination sites.
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
None
ab81669 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"