Recombinant Human Ubiquitin 7-amido-4-methylcoumarin protein (Chemical Free) (ab81665)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P0CG47
    • 生物種Human
    • 分子量9 kDa
    • 配列の追加情報C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC).

特性

Our Abpromise guarantee covers the use of ab81665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

  • 製品の状態Lyophilised
  • 備考

    ab81665 is a fluorogenic substrate for ubiquitin hydrolases. Ubiquitin-AMC is an exquisitely sensitive substrate for UCH-L3 (Km = 0.039 µM) and for the Isopeptidase-T (Km = 0.17 - 1.4 µM). Ub-AMC is useful for studying ubiquitin hydrolases when detection sensitivity or continuous monitoring of activity is essential.

     

    Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 100% DMSO

関連情報

  • 別名
    • Ub-AMC
    • Ubiquitin-AMC
  • 関連性Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
  • 細胞内局在Cytoplasmic and Nuclear

Recombinant Human Ubiquitin 7-amido-4-methylcoumarin protein (Chemical Free) (ab81665) 使用論文

ab81665 has not yet been referenced specifically in any publications.

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