Recombinant Human TrkC protein (Fc Chimera) (ab85599)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号Q16288
    • 生物種Human
    • 配列Theoretical Sequence: CPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNANINITDISRN ITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHL RYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGE AKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVI TCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNG FTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNP PPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLI AKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEEDG SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFF LYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • 領域1 to 428
    • 配列の追加情報Fused with the Fc region of Human IgG1 at the C-terminus.

特性

Our Abpromise guarantee covers the use of ab85599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS

  • 再構成Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • EC 2.7.10.1
    • ETS related protein neurotrophic receptor tyrosine kinase fusion
    • ETS related protein neurotrophic receptor tyrosine kinase fusion protein
    • ETV6 NTRK3 fusion
    • GP145 TrkC
    • gp145(trkC)
    • GP145-TrkC
    • GP145TrkC
    • Neurotrophic tyrosine kinase receptor type 3
    • Neurotrophin 3 receptor
    • NT 3 growth factor receptor
    • NT 3 growth factor receptor precursor
    • NT 3 receptor
    • NT-3 growth factor receptor
    • Ntrk3
    • NTRK3_HUMAN
    • OTTHUMP00000192915
    • TRK C
    • Trk-C
    • TRKC
    • TrkC tyrosine kinase
    • Tyrosine kinase receptor C
    see all
  • 機能Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
  • 組織特異性Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Ligand-mediated auto-phosphorylation.
  • 細胞内局在Membrane.
  • Information by UniProt

Recombinant Human TrkC protein (Fc Chimera) 画像

  • 1D SDS-PAGE of ab85599 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab85599
    Lane 2: ab85599 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab85599 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    5 mg of protein was loaded per lane. Gel was stained with Deep Purple™.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of bands after treatment with glycosidase cocktail suggests the presence of O-linked glycans.

  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.

Recombinant Human TrkC protein (Fc Chimera) (ab85599) 使用論文

ab85599 has not yet been referenced specifically in any publications.

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