製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P54274-2
    • 生物種Human
    • 分子量77 kDa including tags
    • 領域1 to 419

特性

Our Abpromise guarantee covers the use of ab116406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • 質量分析
    MALDI-TOF-TOF
  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituent: 99% PBS

関連情報

  • 別名
    • hTRF1 AS
    • NIMA interacting protein 2
    • NIMA-interacting protein 2
    • PIN 2
    • PIN2
    • t TRF1
    • Telomeric protein Pin2
    • Telomeric protein Pin2/TRF1
    • Telomeric repeat binding factor (NIMA interacting) 1
    • Telomeric repeat binding factor 1
    • Telomeric repeat binding protein 1
    • Telomeric repeat-binding factor 1
    • TERF 1
    • Terf1
    • TERF1_HUMAN
    • TRBF 1
    • TRBF1
    • TRF
    • TRF 1
    • TTAGGG repeat binding factor 1
    • TTAGGG repeat-binding factor 1
    see all
  • 機能Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • 組織特異性Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • 配列類似性Contains 1 HTH myb-type DNA-binding domain.
  • ドメインThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • 翻訳後修飾Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt

Recombinant Human TRF1 protein 画像

  • 10% SDS-PAGE stained with Coomassie Blue (Lane 1), immunobloting with anti-6xHis (Lane 2)

Recombinant Human TRF1 protein (ab116406) 使用論文

ab116406 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116406.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"