Recombinant human Transferrin protein (ab83560)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • 生物種Human
    • 配列Theoretical sequence: VPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASY L DCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKED PQTF YYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLL YCDLPEP RKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCS TLNQYFGYSG AFKCLKNGAGDVAFVKHSTIFENLANKADRDQYEL LCLDNTRKPVDEY KDCHLAQVPSHTVVARSMGGKEDLIWELLNQA QEHFGKDKSKEFQLFS SPHGKDLLFKDSAHGFLKVPPRMDAKMYL GYEYVTAIRNLREGTCQEA PTDECKPVKWCALSHHERLKCDEWSV NSVGKIECVSAETTEDCIAKIM NGEADAMSLDGGFVYIAGKCGLV PVLAENYNKSDNCEDTPEAGYFAVA VVKKSASDLTWDNLKGKKSC HTAVGRTAGWNIPMGLLYNKINHCRFDE FFSEGCAPGSKKDSSLC KLCMGSGLNLCEPNNKEGYYGYTGAFRCLVE KGDVAFVKHQTVPQ NTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEY ANCHLARAPNH AVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLF RSETKDLL FRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSL LEACT FRRP

特性

Our Abpromise guarantee covers the use of ab83560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The activity of ab83560 was measured by its ability to support the growth of HepG2 cells under conditions of reduced serum. Typically 0.1 to 1 µg enhances cell proliferation.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.

関連情報

  • 別名
    • Apotransferrin
    • Beta 1 metal binding globulin
    • Beta-1 metal-binding globulin
    • DKFZp781D0156
    • PRO1400
    • PRO1557
    • PRO2086
    • Serotransferrin
    • Serotransferrin precursor
    • Siderophilin
    • TF
    • TFQTL1
    • Transferin
    • Transferrin
    • TRFE_HUMAN
    see all
  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant human Transferrin protein 画像

  • Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.
  • Lane 1 – MW markers; Lane 2 – ab83560; Lane 3 – ab83560 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83560 treated with a glycosidase cocktail to remove potential N- and Olinked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.
    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
  • A sample of ab83560 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris HCl 2D gel. Approximately 40 µg of protein was load; Gel was stained using Deep Purple™. Spot train indicates presence of multiple isoforms of Transferrin. Spots within the spot train were cut from the gel and identified as Transferrin by protein mass fingerprinting.
  • Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates that ab83560 exists in multiple isoforms, which differ according to their level of posttranslational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW of the protein.

Recombinant human Transferrin protein (ab83560) 使用論文

ab83560 has not yet been referenced specifically in any publications.

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