Recombinant Human TPX2 protein (ab119735)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
製品の詳細
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製品名
Recombinant Human TPX2 protein -
精製度
> 80 % SDS-PAGE.
The purity was determined to be 80% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
110 kDa including tags -
領域
1 to 747 -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab119735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.7% Sodium phosphate, 0.003% DTT, 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride
関連情報
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別名
- C20ORF1
- C20orf2
- Chromosome 20 Open Reading Frame 1
see all -
機能
Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. -
組織特異性
Expressed in lung carcinoma cell lines but not in normal lung tissues. -
配列類似性
Belongs to the TPX2 family. -
発生段階
Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab119735 は論文での使用が確認できていません。