Recombinant Human TPA Tissue Plasminogen Activator protein (FITC) (ab92679)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    CHO cells
  • アミノ酸配列
    • 生物種
      Human
  • 標識
    FITC. Ex: 493nm, Em: 528nm

特性

Our Abpromise guarantee covers the use of ab92679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    It can form 100% complex formation with PAI1.
  • アプリケーション

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.
    >85 percent single chain.
  • 製品の状態
    Liquid
  • 備考
    Protect from light.It can form 100% complex formation with PAI1.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.4M HEPES, 0.1M Sodium chloride, pH 7.4

関連情報

  • 別名
    • Alteplase
    • DKFZp686I03148
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t PA
    • T Plasminogen Activator
    • t-PA
    • T-plasminogen activator
    • Tissue plasminogen activator (t PA)
    • Tissue type plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA_HUMAN
    • TPA1
    see all
  • 機能
    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 組織特異性
    Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 関連疾患
    Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 配列類似性
    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • ドメイン
    Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻訳後修飾
    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 細胞内局在
    Secreted > extracellular space.
  • Information by UniProt

Recombinant Human TPA Tissue Plasminogen Activator protein (FITC) 画像

  • Staining of TPA Tissue Plasminogen Activator protein by 10% SDS-PAGE (UV trans-illuminator).
    Lane 1: ab92679 (3 µg) reduced
    Lane 2: ab92679 (3 µg) non-reduced
  • Staining of TPA Tissue Plasminogen Activator protein by 10% SDS-PAGE.
    Lane 1: ab92679 (3 µg) reduced
    Lane 2: ab92679 (3 µg) non-reduced
    Lane 3: Prestained standard

Recombinant Human TPA Tissue Plasminogen Activator protein (FITC) (ab92679) 使用論文

ab92679 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Immunocytochemistry
tPA-FITC ab92679 was tested in an in vitro uptake assay performed on astrocytes isolated from P2-P3 mouse pups. The astrocytes were cultured in 4 well plates coated with PDL at a density of 25.000 cells/well in DMEM, 10% horse serum and 1% antibiotica (P/S).
Before tPA application, the astrocytes were washed 2 times with pre-warmed DMEM to wash out the horse serum.
After the washes, 140 nM tPA-FITC in DMEM was applied for 15 minutes at 37 degrees. Afterwards, the cells were washed 2 times with 1xPBS and subsequently fixed with 4% PFA at room temperature.
For the ICC, after fixation, the cells were washed 2 times with 1xPBS, blocked with 1xPBS-Triton-X containing 10% NGS (goat serum) and incubated with a GFAP antibody (to visualise the astrocytes) for 1 hour at room temperature. Afterwards, the cells were washed 2 times with 1xPBS and the secondary antibody was applied together with DAPI to visualise the cell nuclei and incubated for 30 min at room temperature in the dark. After washing 3 times with 1xPBS, embedding medium was applied.
For control conditions, some cells were incubated with 140 nM tPA-FITC in DMEM for 15 minutes at 4 degrees (cells do not uptake tPA in these conditions) and some cells with 140 nM Natural Human Serum Albumin protein-FITC (ab8030) in DMEM for 15 minutes at 37 degrees.

The uploaded image shows wild type mouse astrocytes incubated with 140nM tPA for 15 minutes at 37 degrees. Scale bar is 20µm.
Username

Abcam user community

Verified customer

投稿 May 09 2017

Application
Other

Review text: Prior to using for fluoresecent confocal microscopy ab92679 (FITC-tPA) was tested for for fibrinolytic activity by measuring clot lysis using a turbidity assay. Clot lysis was observed with 20 pM ab92679.
For observation of lysis by fluorescent confocal microscopy 75 nM ab92679 was used. A signal could be detected as expected at the lysis front.
Sample: Human Recombinant protein

Primary antibody (in addition to 'TPA Tissue Plasminogen Activator protein (FITC)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
Username

Dr. Claire Whyte

Verified customer

投稿 Nov 27 2012

Thank you for sending the images.
They are now added and I also would like to confirm that the discount codes are active and ready to be used for a further purchase.
The reviews will be allocated to one of team soon and then will soon be publ...

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Thank you for your email Please excuse the late response, Cathrin is not in the office at the moment.
I am happy to confirm that I added the discount codes to the internal notes in the reviews and therefore the codes are no activated.
If you ...

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I am very pleased to hear you would like to accept our offer and test ab92679 and ab93152 in Functional Studies. This code will give you: 1 free product per code before the expiration date. To redeem this offer, please submit an Abreview for Funct...

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Thank you very much for your interest in our products.
As we haven't tested this ourself, it is hard to predict if these proteins will work in a functional study. Therefore, I can offer a discount off a future purchase if you buy ab92679 and ab931...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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