Recombinant human TPA Tissue Plasminogen Activator protein (ab92633)



  • 由来
  • 由来
    CHO cells
  • アミノ酸配列
    • 生物種


Our Abpromise guarantee covers the use of ab92633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific activity: 593,600 IU/mg (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714).
  • アプリケーション

    Functional Studies


  • 精製度
    > 95 % SDS-PAGE.
    ab92633 is > 98% pure by SDS-PAGE.
  • 製品の状態
  • Concentration information loading...


  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.4M HEPES, 0.1M Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 別名
    • Alteplase
    • DKFZp686I03148
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t PA
    • T Plasminogen Activator
    • t-PA
    • T-plasminogen activator
    • Tissue plasminogen activator (t PA)
    • Tissue type plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA1
    see all
  • 機能
    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 組織特異性
    Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 関連疾患
    Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 配列類似性
    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • ドメイン
    Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻訳後修飾
    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 細胞内局在
    Secreted > extracellular space.
  • Information by UniProt


  • 10% SDS-PAGE
    Lane 1: TPA Tissue Plasminogen Activator (3ug) Reduced
    Lane 2: TPA Tissue Plasminogen Activator (3ug) + PAI-1 Reduced (10ug)
    Lane 3: TPA Tissue Plasminogen Activator (3ug) Non-reduced
    Lane 4: TPA Tissue Plasminogen Activator (3ug) + PAI-1 Non-reduced (10ug)
    Lane 5: Prestained Standard


ab92633 has not yet been referenced specifically in any publications.

レビューと Q&A

Please dilute with 0.4 M Hepes, 0.1 M NaCl, pH 7.4. Concentration should be no lower than 100 ug/ml to prevent loss of protein. Azide is compatible with this product.

Thank you for contacting us.

The lab got back to me with the following information:

The endotoxin level of these 2 products cannot be determined because they interfere with the LAL endotoxin clotting assay.

I hope this inform...

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Thank you for contacting us. Our human TPA ab92633 and mouse TPA ab92715 are both suitable for activating the clotting system. The specific activity for these products has been determined using a tPA chromogenic substrate. I hope this helps, please let...

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