Recombinant human TPA Tissue Plasminogen Activator protein (ab68107)



  • 由来Recombinant
  • 由来CHO cells
  • アミノ酸配列
    • 生物種Human


Our Abpromise guarantee covers the use of ab68107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Tissue Plasminogen Activator Human Recombinant (ab68107) produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. Enzymatic Activity: 580,000 IU/mg.
  • アプリケーション


  • 精製度> 95 % SDS-PAGE.
    ab68107 was purified by proprietary chromatographic techniques. Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 製品の状態Lyophilised
  • 備考Upon reconstitution this protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...


  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Each mg of t-PA contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute in sterile 18M Ohms/cm water to not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.


  • 別名
    • Alteplase
    • DKFZp686I03148
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t PA
    • T Plasminogen Activator
    • t-PA
    • T-plasminogen activator
    • Tissue plasminogen activator (t PA)
    • Tissue type plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA1
    see all
  • 機能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 組織特異性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 関連疾患Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • ドメインBoth FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻訳後修飾The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt

Recombinant human TPA Tissue Plasminogen Activator protein (ab68107) 使用論文

ab68107 has not yet been referenced specifically in any publications.

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