Recombinant human Topoisomerase I protein (ab3808)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus
  • アミノ酸配列
    • 生物種Human
    • 分子量91 kDa

特性

Our Abpromise guarantee covers the use of ab3808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    A unit of topo I can relax 0.2 ug pGEM 5Z DNA in 30 min at 37oC. The enzyme is supplied at a nominal concentration of approximately 2 units/ul.

  • アプリケーション

    Functional Studies

    Western blot

  • 製品の状態Liquid
  • 備考

    Quality Control Tests: 1. A test for nuclease contamination is carried out by assaying for the formation of linear KDNA and linear plasmid DNA. Incubation with KDNA or supercoiled pGEM 5Z DNA (4 hrs. at 37ºC in the presence of 10 mM MgCl2) is performed. Linear DNA or breakdown products are not generated under these conditions. 2. A check for cross contamination with Topo II is negative. There must be no decatenation of KDNA in topo II reaction conditions. 3. Performance in converting super-coiled DNA to relaxed DNA.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • DNA topoisomerase 1
    • DNA topoisomerase I
    • NUP98 fusion gene
    • TOP 1
    • TOP I
    • TOP1
    • TOP1_HUMAN
    • TOPI
    • Topoisomerase (DNA) I
    • Topoisomerase 1
    • Topoisomerase1
    • TopoisomeraseI
    • Type I DNA topoisomerase
    see all
  • 機能The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • 関連疾患Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • 配列類似性Belongs to the eukaryotic type I topoisomerase family.
  • 翻訳後修飾Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • 細胞内局在Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt

Recombinant human Topoisomerase I protein 画像

  • Coomassie Blue staining of purified human DNA topoisomerase protein.
  • A unit of topo I incubated with 0.2 µg pGEM 5Z DNA in 30 min at 37ºC.

Recombinant human Topoisomerase I protein (ab3808) 使用論文

ab3808 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"