製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号Q9UKE5
    • 生物種Human
    • 分子量67 kDa including tags
    • 領域1 to 367
    • タグGST tag N-Terminus

関連製品

特性

Our Abpromise guarantee covers the use of ab119725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性ab119725 specific activity determined to be 68 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    The purity was determined to be 95% by densitometry
  • 製品の状態Liquid
  • 備考

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 25% Glycerol, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 0.003% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • EC 2.7.11.1
    • KIAA0551
    • Tnik
    • TNIK_HUMAN
    • Traf2 and NCK interacting kinase
    • Traf2 and NCK-interacting protein kinase
    see all
  • 機能Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading.
  • 組織特異性Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
  • 細胞内局在Nucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
  • Information by UniProt

Recombinant human TNIK protein 画像

  • SDS Page analysis of ab119725
  • ab119725 specific activity determined to be 68 nmol/min/mg.

Recombinant human TNIK protein (ab119725) 使用論文

ab119725 has not yet been referenced specifically in any publications.

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