Recombinant human TNFRSF14/HVEM protein (Active) (ab219714)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human TNFRSF14/HVEM protein (Active)
TNFRSF14/HVEM タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized ab219714 at 0.5 µg/mL (100 µL/well) can bind Human BTLA, Fc Tag with a linear range of 0.078-2.5 µg/mL.
-
精製度
> 90 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLN GLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAAC RAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWL VTKAGAGTSSSHWV -
予測される分子量
19 kDa including tags -
領域
39 to 202 -
タグ
His tag C-Terminus -
配列の追加情報
(Accession # NP_003811).
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab219714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Lyophilized -
備考
This product was previously labelled as TNFRSF14
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 95% PBS
Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
関連情報
-
別名
- HVEML
- ATAR
- CD270
see all -
機能
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. -
組織特異性
Widely expressed, with the highest expression in lung, spleen and thymus. -
配列類似性
Contains 3 TNFR-Cys repeats. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
画像
-
Immobilized Human HVEM, His Tag at 0.5 µg/mL (100 µL/well) can bind Human BTLA, Fc Tag with a linear range of 0.078-2.5 µg/mL.
-
SDS-PAGE analysis of reduced ab219714 stained overnight with Coomassie Blue.
-
Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin at 2 µg/mL (100 µL/well) can bind Human HVEM, His Tag with a linear range of 0.04-0.6 µg/mL.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab219714 は論文での使用が確認できていません。