The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ND50 of ab83577 is typically 15-30 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D.
Concentration information loading...
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Tumor necrosis factor receptor 1
Tumor necrosis factor receptor superfamily, member 1A
Tumor necrosis factor receptor type 1
Tumor necrosis factor receptor type I
Tumor necrosis factor-binding protein 1
機能Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
関連疾患Familial hibernian fever Multiple sclerosis 5
配列類似性Contains 1 death domain. Contains 4 TNFR-Cys repeats.
ドメインThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE. Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
翻訳後修飾The soluble form is produced from the membrane form by proteolytic processing.
細胞内局在Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
Recombinant human TNF Receptor I protein (Fc Chimera) 画像
Functional Studies - TNF Receptor I protein (Fc Chimera Active) (ab83577)
Densitometry scan demonstrating the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
modification. The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - TNF Receptor I protein (Fc Chimera Active) (ab83577)
1D SDS-PAGE of ab83577 before and after treatment with glycosidases to remove oligosaccharides. Lane 1 – MW markers; Lane 2 – ab83577 ; Lane 3 – ab83577 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83577 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Slight drop in MW after treatment with glycosidase cocktail suggests presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
Recombinant human TNF Receptor I protein (Fc Chimera) (ab83577) 使用論文
has not yet been referenced specifically in any publications.