製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    HEK 293 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 領域
      27 to 220
    • タグ
      His tag C-Terminus

特性

Our Abpromise guarantee covers the use of ab125639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    The IC50 of 3 nM is measured by its ability to inhibit recombinant Human MMP2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.
  • アプリケーション

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.
    Extensive dialysis against PBS.
  • 製品の状態
    Lyophilised
  • 備考
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). The IC50 of 3 nM is measured by its ability to inhibit recombinant Human MMP2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5)

関連情報

  • 別名
    • CSC 21K
    • CSC-21K
    • CSC21K
    • Metalloproteinase inhibitor 2
    • Metalloproteinase inhibitor 2 precursor
    • TIMP 2
    • TIMP metallopeptidase inhibitor 2
    • TIMP-2
    • TIMP2
    • TIMP2_HUMAN
    • Tissue Inhibitor of Metalloproteinase 2
    • Tissue inhibitor of metalloproteinases 2
    see all
  • 機能
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • 配列類似性
    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • 翻訳後修飾
    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • 細胞内局在
    Secreted.
  • Information by UniProt

参考文献

ab125639 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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