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Our Abpromise guarantee covers the use of ab54435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The recombinant mature TIE-2/Fc is a disulfide-linked homodimeric protein. The soluble receptor protein consists of the full extracellular domain (Met1-Val730).
Human TIE-2-Fc monomer has a calculated molecular mass of approximately 105kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125kDa protein in SDS-PAGE under reducing conditions.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
SDS-PAGE of recombinant human soluble TIE-2/Fc produced from insect cells. The sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
ab54435 has not yet been referenced specifically in any publications.