The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
% SDS-PAGE. ab123484 was purified by standard chromatography and filter sterilized.
備考Please prevent freeze thaw cycles.
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Shipped at 4°C. Store at -20°C. Store under Desiccating conditions.
pH: 7.40 Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ATL derived factor
Surface associated sulphydryl protein
Surface-associated sulphydryl protein
機能Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
配列類似性Belongs to the thioredoxin family. Contains 1 thioredoxin domain.
翻訳後修飾In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
細胞内局在Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.