Recombinant human TGF beta 1 protein (ab50038)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来CHO cells
  • アミノ酸配列
    • 生物種Human
    • 配列ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
    • 分子量25 kDa
    • 配列の追加情報ab50038 composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

特性

Our Abpromise guarantee covers the use of ab50038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    The ED50 was determined by TGF beta 1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is < 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

関連情報

  • 別名
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • Prepro transforming growth factor beta 1
    • TGF beta
    • TGF beta 1
    • TGF beta 1 protein
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta-5
    • TGF-beta1
    • TGFB
    • Tgfb-1
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • TGFbeta1
    • Transforming Growth Factor b1
    • Transforming Growth Factor beta 1
    • Transforming growth factor beta 1a
    • transforming growth factor beta-1
    • transforming growth factor, beta 1
    see all
  • 機能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 組織特異性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 関連疾患Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 配列類似性Belongs to the TGF-beta family.
  • 翻訳後修飾Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Recombinant human TGF beta 1 protein (ab50038) 使用論文

ab50038 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I assume you would like to use the protein for functional studies? From the looks of it, all three of them seem to be suitable for Functional Studies, but withinAb50036the endotoxin level has been determined as w...

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The TGF-beta 1 is secreted from the CHO cells, the media is then collected and the protein is isolated and purified. Please do not hesitate to contact me should you have any additional questions.

Thank you for your enquiry.

I have reviewed with our sources the TGF beta 1 proteins that we have on our catalog. It seems the most suitable would be ab50036 TGF beta 1 protein (Active).

http://www.abcam.com/index.html?datasheet=5...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"