Recombinant human TGF beta 1 protein (Active) (ab116150)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human TGF beta 1 protein (Active)
TGF beta 1 タンパク質・ペプチド 製品一覧 -
生理活性
Method of determination: Inhibition of IL-4-induced HT-2 cell proliferation
Acceptance criteria: ED50 ≤500 pg/mL (≥ 2.0 x 10^6 units/mg)
The activity of Human TFG-beta is 2.2 x 10^4 RU/μg, which was calibrated against recombinant human TGF-beta 1.
-
精製度
> 95 % SDS-PAGE.
Protein content and purity was determined by UV spectroscopy at 280 nm; RP-HPLC calibrated against a known standard and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
CHO cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CSALDTNYCF SSTEKNCCVR QLYIDFRKDL GWKWIHEPKG YHANFCLGPC PYIWSLDTQY SKVLALYNQH NPGASAAPCC VPQALEPLPI VYYVGRKPKV EQLSNMIVRS CKCS -
予測される分子量
26 kDa -
領域
279 to 390
-
特性
Our Abpromise guarantee covers the use of ab116150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
HPLC
Functional Studies
SDS-PAGE
-
製品の状態
Lyophilized -
備考
Endotoxin levels determined by LAL method.
For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at Room Temperature. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituents: 0.1% Trifluoroacetic acid, 95% Trehalose
Lyophilized from a sterile (0.2 micron) filtered aqueous solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成ab116150 should be reconstituted with sterile 10 mM HCl at a concentration of 0.1 mg/ml. When reconstituting the product, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
関連情報
-
別名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
機能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
組織特異性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
関連疾患
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
配列類似性
Belongs to the TGF-beta family. -
翻訳後修飾
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab116150 は 1 報の論文で使用されています。
- Malikova AZ et al. Pre-Senescence Induction in Hepatoma Cells Favors Hepatitis C Virus Replication and Can Be Used in Exploring Antiviral Potential of Histone Deacetylase Inhibitors. Int J Mol Sci 22:N/A (2021). PubMed: 33925399