製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号P52294
    • 生物種Human
    • 配列MTTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNV ATAEEETEEEVMSDGGFHEAQINNMEMAPGGVITSDMIEMIFSKSPEQQL SATQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFESAWV LTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDS TMCRDYVLDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFA KVSPCLNVLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRR LVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSP KESIKKEACWTISNITAGNRAQIQTVIDANIFPALISILQTAEFRTRKEA AWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDSKIVQVALNGLENI LRLGEQEAKRNGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLI EHYFGTEDEDSSIAPQVDLNQQQYIFQQCEAPMEGFQL
    • 分子量85 kDa including tags
    • 領域1 to 538

特性

Our Abpromise guarantee covers the use of ab114791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

    ELISA

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 mg/ml.
    This protein is best used within three months from the date of receipt.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • IMA5_HUMAN
    • Importin alpha 1 subunit
    • Importin alpha 5
    • Importin alpha S1
    • Importin subunit alpha-5, N-terminally processed
    • IPO A5
    • IPOA 5
    • IPOA5
    • Karyopherin alpha 1
    • Karyopherin alpha 1 subunit
    • Karyopherin subunit alpha-1
    • KPNA 1
    • KPNA1
    • mSRP 1
    • mSRP1
    • NPI 1
    • NPI-1
    • NPI1
    • Nucleoprotein interactor 1
    • RAG cohort protein 2
    • RCH 2
    • RCH2
    • Recombination activating gene cohort 2
    • SRP 1
    • SRP1 beta
    • SRP1-beta
    see all
  • 機能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • 組織特異性Expressed ubiquitously.
  • 配列類似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • ドメインConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 翻訳後修飾Polyubiquitinated in the presence of RAG1 (in vitro).
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human SRP1 protein 画像

  • ab114791 on 12.5% SDS-PAGE Stained with Coomassie Blue.

Recombinant Human SRP1 protein (ab114791) 使用論文

ab114791 has not yet been referenced specifically in any publications.

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