Recombinant Human Src protein (ab90186)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P12931
    • 生物種Human
    • 領域84 to 145
    • タグGST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab90186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Potassium phosphate, 20mM Sodium chloride, pH 6.5

  • 再構成Reconstitute with in H2O bid.

関連情報

  • 別名
    • ASV
    • Avian sarcoma virus
    • c SRC
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA
    • cSrc
    • EC 2.7.10.2
    • Neuronal CSRC tyrosine specific protein kinase
    • Neuronal SRC
    • Oncogene SRC
    • OTTHUMP00000174476
    • OTTHUMP00000174477
    • p60 Src
    • p60-Src
    • p60Src
    • pp60c src
    • pp60c-src
    • pp60csrc
    • Proto oncogene tyrosine protein kinase Src
    • Proto-oncogene c-Src
    • Proto-oncogene tyrosine-protein kinase Src
    • Protooncogene SRC
    • Protooncogene SRC Rous sarcoma
    • Src
    • SRC Oncogene
    • SRC proto oncogene non receptor tyrosine kinase
    • SRC_HUMAN
    • SRC1
    • Tyrosine kinase pp60c src
    • Tyrosine protein kinase SRC 1
    • Tyrosine protein kinase SRC1
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian)
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian
    see all
  • 機能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 細胞内局在Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt

Recombinant Human Src protein (ab90186) 使用論文

ab90186 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"