製品の概要

製品の詳細

  • 由来Recombinant
  • 由来CHO cells
  • アミノ酸配列
    • アクセッション番号P09486
    • 生物種Human
    • 配列APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVV AENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNK TFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRM RDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLA RDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTT RFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI
    • 分子量44 kDa
    • 領域18 to 303

特性

Our Abpromise guarantee covers the use of ab107952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5-0.7 µg/ml.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 精製度> 98 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10mM Sodium phosphate, pH 7.6

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute in water to a concentration of 0.1-1.0 mg/ml

関連情報

  • 別名
    • AA517111
    • Basement membrane protein 40
    • Basement-membrane protein 40
    • BM 40
    • BM-40
    • BM40
    • Cysteine rich protein
    • hm:zeh0062
    • MGC128090
    • OI17
    • ON
    • Osteonectin
    • Secreted acidic cystein rich glycoprotein
    • Secreted protein acidic and rich in cysteine
    • Secreted protein acidic cysteine rich
    • Secreted protein acidic cysteine rich (osteonectin)
    • SPARC
    • SPRC
    • SPRC_HUMAN
    see all
  • 機能Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • 配列類似性Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • 発生段階Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • 細胞内局在Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt

Recombinant human SPARC protein (ab107952) 使用論文

ab107952 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"