Recombinant human SOD2/MnSOD protein (ab93946)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Human
    • 配列
      MGSSHHHHHHSSGLVPRGSHMKHSLPDLPYDYGALEPHINAQIMQLHHSK HHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFWT NLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFN KERGHLQIAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKA IWNVINWENVTERYMACKK

特性

Our Abpromise guarantee covers the use of ab93946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.

    Activity Assay

    1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochrome C, 0.05mM xanthine, 0.005 units xanthine oxidase.
    2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
    3. Add 50 ul of recombinant SOD protein in various concentrations (0.5 ug, 1 ug) in assay buffer.
    4. Mix by inversion and record the increase at A550nm for 5 minutes.
  • アプリケーション

    SDS-PAGE

    Mass Spectrometry

  • 質量分析
    MALDI-TOF
  • 精製度
    > 95 % SDS-PAGE.
    ab93946 was purified using conventional chromatography techniques.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Indophenoloxidase B
    • IPO B
    • IPOB
    • Manganese containing superoxide dismutase
    • Manganese SOD
    • Manganese superoxide dismutase
    • Mangano superoxide dismutase
    • Mn SOD
    • Mn superoxide dismutase
    • MNSOD
    • MVCD6
    • SOD 2
    • SOD2
    • SODM_HUMAN
    • Superoxide dismutase [Mn] mitochondrial
    • Superoxide dismutase [Mn], mitochondrial
    • Superoxide dismutase 2 mitochondrial
    see all
  • 機能
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性
    Belongs to the iron/manganese superoxide dismutase family.
  • 翻訳後修飾
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt

Recombinant human SOD2/MnSOD protein 画像

  • 15% SDS-PAGE analysis of ab93946 (3µg)

Recombinant human SOD2/MnSOD protein (ab93946) 使用論文

ab93946 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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