Recombinant Human SMYD3 protein (ab80377)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human
    • 領域35 to 428

関連製品

特性

Our Abpromise guarantee covers the use of ab80377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

関連情報

  • 別名
    • bA74P14.1
    • bA74P14.1 (novel protein)
    • FLJ21080
    • histone lysine N methyltransferase SMYD3
    • KMT3E
    • MGC104324
    • SET and MYND domain containing 3
    • SET and MYND domain containing protein 3
    • SET and MYND domain-containing protein 3
    • SMYD 3
    • Smyd3
    • SMYD3 protein
    • SMYD3_HUMAN
    • Zinc finger MYND domain containing 1
    • Zinc finger MYND domain containing protein 1
    • Zinc finger MYND domain-containing protein 1
    • Zinc finger protein subfamily 3A MYND domain containing 1
    • Zinc finger protein, subfamily 3A (MYND domain containing), 1
    • ZMYND 1
    • ZMYND1
    • ZNFN3A1
    see all
  • 機能Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • 組織特異性Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • 配列類似性Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • 細胞内局在Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt

Recombinant Human SMYD3 protein 画像

  • Lane 1: ab80377 on 10% SDS-PAGE, Coomassie staining, 4µg.
    Lane 2: Protein marker.

Recombinant Human SMYD3 protein (ab80377) 使用論文

ab80377 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your response.

We currently do not know of an accurate method to accurately determine the specific activity of SMYD3 enzyme. We are still in contact with the lab and will let you know should this information become available. ...

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Thank you for your email.

Unfortunately we do not currently have any antibodies directed towards the methylated K5 of histone 4. We only have antibodies to the acetylated targetof thisresidue (rabbit polyclonals http://www.abcam.com/Histone-H...

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Thank you for contacting us.

Unfortunately I have been informed that the fluorescence polarisation assay described on the datasheet of SMYD3 protein (Active) (ab80377) is not an accurate assay to determine the activity of SMYD3. We will be up...

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Thank you contacting us with these further questions. I have spoken with the lab about a usefull concentration for the purified HMT enzyme. Based on tests, we conclude that the useful concentration range can be from 10 ng to 200 ng/well (30 ul). ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"