製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Human

特性

Our Abpromise guarantee covers the use of ab84653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5

関連情報

  • 別名
    • BSP-1
    • BSP1
    • HsMAD1
    • JV4-1
    • JV41
    • MAD homolog 1
    • MAD mothers against decapentaplegic homolog 1
    • Mad related protein 1
    • Mad-related protein 1
    • MADH1
    • MADR1
    • Mothers against decapentaplegic homolog 1
    • Mothers against DPP homolog 1
    • SMA- AND MAD-RELATED PROTEIN 1
    • SMAD 1
    • SMAD family member 1
    • SMAD mothers against DPP homolog 1
    • Smad1
    • SMAD1_HUMAN
    • TGF beta signaling protein 1
    • Transforming growth factor-beta-signaling protein 1
    see all
  • 機能
    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • 組織特異性
    Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • 配列類似性
    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • 翻訳後修飾
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt

画像

  • SDS-PAGE showing ab84653 at approximately 83kDa.
  • Anti-Smad1 antibody (ab46688) at 1 µg/ml + Recombinant Human Smad1 protein (ab84653) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

参考文献

ab84653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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