Recombinant human SIRT3 protein (Tagged) (ab54333)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      59 kDa
    • 領域
      102 to 399
    • タグ
      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab54333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Specific Activity: 6 U/ug. One Unit =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM BPS HDAC substrate , 200 uM NAD+, and 20 ng/ul Sirtuin 3. Incubation condition: 30 min at 30ºC.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度
    > 90 % SDS-PAGE.

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 20mM Glutathione, 138mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • hSIRT 3
    • hSIRT3
    • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase
    • NAD dependent deacetylase sirtuin 3 mitochondrial
    • NAD-dependent protein deacetylase sirtuin-3, mitochondrial
    • Regulatory protein SIR2 homolog 3
    • Silent mating type information regulation 2 S.cerevisiae homolog 3
    • Sir 2 like 3
    • SIR 2 like protein 3
    • SIR 3
    • SIR2 L3
    • Sir2 like 3
    • SIR2 like protein 3
    • SIR2-like protein 3
    • SIR2L3
    • SIR3_HUMAN
    • SIRT 3
    • SIRT3
    • Sirtuin 3
    • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae)
    • Sirtuin type 3
    • Sirtuin3
    see all
  • 機能
    NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 翻訳後修飾
    Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt

参考文献

ab54333 has not yet been referenced specifically in any publications.

レビューと Q&A

Cette protéine est une protéine humaine de Sirtuin 3 et consiste des acides aminés 102-399 avec un tag GST au N terminale.

Le poids moléculaire de cette protéine devrait donc être de 59 kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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