Recombinant human SIRT3 protein (ab125810)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human SIRT3 protein
SIRT3 タンパク質・ペプチド 製品一覧 -
生理活性
The specific activity of ab125810 was determined to be 150 RLU/min/mg.
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精製度
> 70 % Densitometry.
Purity was determined to be >70% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
66 kDa including tags -
領域
47 to 399
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特性
Our Abpromise guarantee covers the use of ab125810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- hSIRT 3
- hSIRT3
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase
see all -
機能
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain. -
翻訳後修飾
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab125810 は 1 報の論文で使用されています。
- Nadtochiy SM et al. Potential mechanisms linking SIRT activity and hypoxic 2-hydroxyglutarate generation: no role for direct enzyme (de)acetylation. Biochem J 474:2829-2839 (2017). PubMed: 28673962